|
KMID : 1025520120540020103
|
|
Journal of Animal Science and Technology
2012 Volume.54 No. 2 p.103 ~ p.109
|
|
|
Comparison between Single and Co-culture of Adipocyte and Muscle Cell Lines in Cell Morphology and Cytosolic Substances
|
|
Choi Chang-Weon
Cho Won-Mo
Yeon Seong-Heum
Hwangbo Soon
Song Man-Kang
Park Sung-Kwon
Baek Kyung-Hoon
|
|
|
Abstract
|
|
|
|
Present study was performed to investigate the effect of single and co-culture of adipocyte and muscle cell lines on cell differentiation. 3T3-L1(adipocyte) and L6(muscle) cell lines were single-cultured on the condition of 10% fetal bovine serum (FBS)/Dulbeco’s modified eagle’s medium(DMEM) for 48 h followed by culture within 5% FBS/DMEM as a growth media. Then, the growth media was replaced by differentiation media composed of 2% FBS/DMEM without additives in single- or co-culture of the 3T3-L1 and the L6 cells to induce differentiation of both cell types. In co-culture system, the 3T3-L1 and the L6 cells were grown in separated places by being seeded on a 0.4μm insert membrane and on the bottom of 6 well plate, respectively. Cell differentiation was measured using morphological investigation and cytosolic analysis of glycerol-3-phosphate dehydrogenase(GPDH; for 3T3-L1) and creatine kinase(CK; for L6). Based on the GPDH results, the presence of L6 cells did not stimulate 3T3-L1 differentiation showing more differentiation of 3T3-L1 cells in the single-culture compared to the co-culture condition. In contrast, 3T3-L1 cells in the co-culture promoted differentiation of L6 cells. Enzymatic analysis supported this result showing that 3T3-L1 cells showed statistically(P<0.05) higher GPDH activity in the single-culture than the co-culture, whereas CK results of L6 cells were vice versa(P<0.05). Overall, present results may indicate that co-culture system is more reliable and precise technique compared to single-culture. Further studies on several co-culture trials including different media conditions, supplementation of differentiating substances, molecular biological analysis, etc. should be required to obtain practical and fundamental mass data.
|
|
|
KEYWORD
|
|
|
Cell line, Co-culture, Adipocyte, Muscle cells
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|